We assess the empirical validity this theoretical proposition by comparing the same respondents’ opinions across two waves of a large-scale survey conducted within the European Commission in 2008 and 2014. Identification of leadership effects on Commission’s AD-level staff derives from changes over the 2009-2014 period in top political (i.e. Commissioners) and administrative (i.e. Directors-General) leadership positions within the Commission. Our main findings show that a Commissioner from a more Eurosceptic country causes a statistically significant decline in AD-level staff’s internationalist attitudes. It also has a negative effect on staff members’ opinion that decision-making authority in specific policy areas should lie with the Commission rather than the Member States. No similar significant effects are observed for changes in Directors-General, which is consistent with the important institutional differences between both types of leadership positions.